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TaqMan miRNA定量PCR试剂盒

产品编号 产品名称 规格 价格(元) 说明书
TAPXXXXX HG TaqMan miRNA定量PCR试剂盒 100次×20μl 600
  请于此文件中查询对应试剂盒货号TaqMan miRNA qPCR kit.xls
探针法做为microRNA定量研究的金标准,其具有高特异性、高灵敏度的优势。HaiGene的HG TaqMan miRNA定量PCR试剂盒,采用特异性的正向miRNA引物和接头引物进行PCR扩增,检测荧光基团采用FAM标记的TaqMan探针。使用该试剂盒可以高特异性、高灵敏度的检测低至100个细胞的miRNA分子,并可区分相似度极高的miRNAs。HaiGene的TaqMan miRNA定量PCR试剂盒多达一万余种,试剂盒编号为TAPxxxxx,每一个mircoRNA分子对应一个检测试剂盒(包含特异性的TaqMan探针、正向引物、反向引物、定量试剂),可于HG TaqMan miRNA qPCR kit.xls中查询。
特别说明:如您研究的miRNA不在我们的TaqMan miRNA qPCR Kit列表之中,请登录www.mirbase.org进行miRNA查询,并来信告知Accession No.和序列,我们将在2周内完成试剂盒的定制。

原理

taqMan microRNA定量PCR检测

组分

试剂盒组分名称 数量(100T×20 μl)
5×Golden HS TaqMan qPCR Mix 400 μl
50×ROX Reference Dye 200 μl
20×miRNA TaqMan Assay 100 μl

储存

避光置于-20°C,可保存2年;避免反复冻融。

检测效果和应用实例

1. 高灵敏度 (最高可到0.1fM级水平)
2.高特异性,有效区分单碱基差异
3. 应用实例
注:其它miRNA荧光定量检测试剂盒请查询HG TaqMan miRNA qPCR kit.xls

参考文献

[1] Development of a robust, low cost stem-loop real-time quantification PCR technique for miRNA expression analysis. Mol Biol Rep. 2013. PMID: 23307300.
[2] Quantitative stem-loop RT-PCR for detection of microRNAs. Methods Mol Biol. 2011. PMID: 21533691.
[3] MicroRNA detection in prostate tumors by quantitative real-time PCR (qPCR). J Vis Exp. 2012. PMID: 22643910.
[4] Circulating microRNA-196a as a candidate diagnostic biomarker for chronic hepatitis C. Mol Med Rep. 2015. PMID: 25738504.
[5] Novel real-time PCR assay of microRNAs using S-Poly(T), a specific oligo(dT) reverse transcription primer with excellent sensitivity and specificity. PLoS One. 2012. PMID: 23152780.
[6] Diagnostic microRNA markers to screen for sporadic human colon cancer in stool: I. Proof of principle. Cancer Genomics Proteomics. 2013. PMID: 23741026.
[7] Stem-loop RT-qPCR for microRNA expression profiling. Methods Mol Biol. 2012. PMID: 22144190.
[8] Serum microRNA-21 as marker for necroinflammation in hepatitis C patients with and without hepatocellular carcinoma. PLoS One. 2011. PMID: 22066022.