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产品编号
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产品名称
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规格
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价格(元)
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说明书
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A3901
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Bst 3.0 DNA/RNA Polymerase(8U/ul)50%甘油
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1600U
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500
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16KU
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3,750
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与Bst 2.0 DNA 聚合酶和Bst DNA 聚合酶大片段相比，Bst 3.0 DNA聚合酶具有更快的等温扩增速度和更强的逆转录活性。无论以DNA还是RNA为模板，该酶都具有5’-3’的DNA聚合酶活性和强烈的链置换活性，但该酶5’-3’和3’-5’的外切酶活性缺失。在以RNA为模板的LAMP实验中，可实现单酶系统反应。Bst 3.0 DNA聚合酶在60-65℃之间具有很好的反转录活性，可有效解决具有二级复杂结构的RNA模板的反转录，而Bst 2.0 DNA 聚合酶和Bst DNA 聚合酶大片段无此活性。
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Bst2.0
(A3801)
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Bst3.0
(A3901)
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Bst4.0
(A3805)
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热启动Bst4.2
(A3831-00)
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热启动Bst dU5.0
(A3861-00)
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热启动功能
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否
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否
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否
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是
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是
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酶储存液
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50%甘油
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50%甘油
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50%甘油
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无甘油
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无甘油
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反应温度
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65℃
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65℃
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65℃
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70℃
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65~70℃
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检测底物
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DNA
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DNA
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DNA/RNA
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DNA/RNA
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DNA/RNA
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dUTP使用能力
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50%
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50%
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50%
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50%
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100%
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扩增速度
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★★★
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★★★★★
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★★★★★
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★★★★★
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★★★★
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LAMP Mix供货
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不提供
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不提供
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不提供
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A3831-20基础预混液(dNTP)
A3831-21荧光预混液(dNTP)
A3831-22变色预混液(dNTP)
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A3861-20基础预混液(全dUTP)
A3861-21荧光预混液(全dUTP)
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LAMP冻干品供货
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不提供
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不提供
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不提供
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A3831-56变色冻干球(dNTP)
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A3861-22基础冻干瓶(全dUTP)
A3861-23荧光冻干瓶(全dUTP)
A3861-24基础冻干球(含UDG酶)
A3861-25荧光冻干球(含UDG酶)
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单位定义
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1U是指在65°C条件下，30分钟内催化10nmol dNTP的掺入反应，成为酸不溶性物质所需的酶量。
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酶储存液
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10mM Tris-HCl, 150mM KCl, 2mM DTT, 0.1mM EDTA, 50%Glycerol, pH7.5。
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热失活
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85°C，5min。
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性能展示
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Bst3.0-4.0 RNA模板的扩增能力展示:RNA模板的扩增，分别用Bst 2.0、3.0、4.0以A549细胞RNA为模板，进行RT-LAMP扩增。
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应用LAMP产品文献
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1. Rapid Visual Detection of Elite Erect Panicle Dense and Erect Panicle 1 Allele for Marker-Assisted Improvement in Rice (Oryza sativa L.) Using the Loop-Mediated Isothermal Amplification Method. Curr Issues Mol Biol. 2024 Jan 4;46(1):498–512.
2. Rapid Visual Detection of High Nitrogen-Use Efficiency Gene OsGRF4 in Rice (Oryza sativa L.) Using Loop-Mediated Isothermal Amplification Method. Genes (Basel). 2023 Sep 23;14(10):1850.
3. Rapid and visual identification of HIV-1 using reverse transcription loop-mediated isothermal amplification integrated with a gold nanoparticle-based lateral flow assay platform. Front Microbiol. 2023 Jul 12;14:1230533.
4. Rapid Detection of Streptococcus mutans Using an Integrated Microfluidic System with Loop-Mediated Isothermal Amplification. J Microbiol Biotechnol. 2023 May 19;33(8):1101–1110.
5. Rapid point-of-care detection of BK virus in urine by an HFman probe-based loop-mediated isothermal amplification assay and a finger-driven microfluidic chip.PeerJ. 2023 Mar 8;11:e14943.
6. Rapid detection of monkeypox virus and monkey B virus by a multiplex loop-mediated isothermal amplification assay. J Infect. 2023 Feb 13;86(4):e114–e116.
7. SMART: A Swing-Assisted Multiplexed Analyzer for Point-of-Care Respiratory Tract Infection Testing. Biosensors (Basel). 2023 Feb 4;13(2):228.
8. An HFman Probe-Based Multiplex Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Simultaneous Detection of Hantaan and Seoul Viruses. Diagnostics (Basel). 2022 Aug 10;12(8):1925.
9. Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method. Biosensors (Basel). 2022 Apr 3;12(4):213.
10.Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe.ACS Sens. 2022 Feb 22;7(3):730–739.
11.Deep‐dLAMP: Deep Learning‐Enabled Polydisperse Emulsion‐Based Digital Loop‐Mediated Isothermal Amplification. Adv Sci (Weinh). 2022 Jan 24;9(9):2105450.
12.Development of a TaqMan loop-mediated isothermal amplification assay for the rapid detection of pigeon paramyxovirus type 1. Arch Virol. 2021 Mar 23;166(6):1599–1605.
13.Rapid, visual, label-based biosensor platform for identification of hepatitis C virus in clinical applications. BMC Microbiol. 2024 Feb 28;24:68.
14.A novel extraction-free dual HiFi-LAMP assay for detection of methicillin-sensitive and methicillin-resistant Staphylococcus aureus.Microbiol Spectr. 2024 Feb 20;12(4):e04133-23.
15.A duplex HiFi-LAMP assay for screening of two novel human circoviruses HCirV-1 and HCirV-2. BMC Infect Dis. 2024 Dec 5;24:1388.
16.Point-of-care test of blood Plasmodium RNA within a Pasteur pipette using a novel isothermal amplification without nucleic acid purification. Infect Dis Poverty. 2024 Oct 31;13:80.
17.Rapid detection of human influenza A viruses by HFman probe-based loop-mediated isothermal amplification assays. Heliyon. 2023 Oct 25;9(11):e21591.
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