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miRNA荧光定量PCR试剂盒(SYBR Green)

产品编号 产品名称 规格 价格(元) 说明书
APXXXXX HG SYBR Green miRNA荧光定量PCR试剂盒 100次 500
  请于此文件中查询对应试剂盒货号HG SRBR Green miRNA qPCR kit.xls

HG SYBR Green miRNA荧光定量PCR试剂盒应用Peter改良的miR-Q技术,采用特异性的上下游引物对反转录所得miRNA的cDNA产物进行定量检测【1】。不同于其它公司的单条特异性引物扩增方法,HaiGene采用双向特异性扩增引物(原理如图1),从而极大提高了miRNA的检测特异性,其可以区分极高相似度的miRNA分子、减少非特异性扩增(图2,3所示)。该miRNA荧光定量PCR试剂盒对可以对任何miRNA分子进行检测。HG miRNA荧光定量PCR试剂盒共有一万余种,试剂盒编号为APXXXXX,每一个miRNA分子对应一个检测试剂盒,可于HG SYBR Green miRNA qPCR kit.xls中查询。如果您研究的miRNA分子不在我们的列表中,请来信咨询,我们将及时给您优化设计。该试剂盒中的每一对引物均经过设计优化,确保扩增效率和特异性。使用该方法进行miRNA定量检测已被大量文献所采用【2~11】

miRNA荧光定量PCR试剂盒
miRNA荧光定量PCR试剂盒

组分

试剂盒组分名称 数量
2×Hi SYBR Green qPCR Mix 1 ml
50×ROX Reference Dye 200 μl
miRNA Primer F(10μM) 100 μl
miRNA Primer R(10μM) 100 μl
miRNA 标准品(1pM) 100 μl

储存

避光置于-20°C,可保存2年;避免反复冻融。

常用miRNA内参荧光定量PCR试剂盒

产品编号 产品名称 规格 价格(元)
AP01501 HG SYBR Green RNU6B miRNA荧光定量PCR试剂盒
(适用于组织和细胞样本)
100T×20μl 500
AP01511 HG SYBR Green miR-16 miRNA荧光定量PCR试剂盒
(适用于全血样本)
100T×20μl 500
注:其它miRNA荧光定量检测试剂盒请查询HG SYBR Green miRNA qPCR kit.xls

参考文献

(其中3-11为使用该方法发表的文献)
[1] Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers. BMC Biotechnol. 2011 Jun 25; 11: 70. PMC3135530.
[2] Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling. J Anim Sci Biotechnol. 2012 PMID:22958414.
[3] Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans. BMC Genomics. 2014. PMID:24656064.
[4] Profiling microRNAs in lung tissue from pigs infected with Actinobacillus pleuropneumoniae. BMC Genomics. 2012. PMID:22953717.
[5] The role of viral and host microRNAs in the Aujeszky's disease virus during the infection process. PLoS One. 2014. PMID:24475202.
[6] MicroRNA polymorphisms and environmental smoke exposure as risk factors for oesophageal squamous cell carcinoma. PLoS One. 2013.PMID:24205249.
[7] Plasma proANP and SDMA and microRNAs are associated with chronic mitral regurgitation in a pig model. Endocr Connect. 2013.PMID:24029364.
[8] Advances in microRNA experimental approaches to study physiological regulation of gene products implicated in CNS disorders. Exp Neurol. 2012. PMID:22245616.
[9] Maternally deposited germline piRNAs silence the tirant retrotransposon in somatic cells. EMBO Rep. 2013. PMID:23559065.
[10] miRNA expression profile analysis in kidney of different porcine breeds. PLoS One. 2013. PMID:23372853.
[11] Determination of reference microRNAs for relative quantification in porcine tissues. PLoS One. 2012. PMID:22970213.