超敏ECL发光液|新海基因检测有限公司

超敏ECL发光液

产品编号	产品名称	规格	价格(元)	说明书
M2301	超敏ECL化学发光液	50ml/500ml	300/2,000

Western Blot结果的好坏与否与ECL发光液的选择有着密切关系，好的ECL发光液不仅可曝光得到漂亮的目的条带，还可节省昂贵的抗体。海基超敏ECL发光液用于Western、Northern和Southern blot分子杂交的化学发光检测，原理是采用新型发光底物（Luminol），其与辣根过氧化物酶反应时产生很强的发光信号，以此检测微量蛋白而获得理想的实验结果。比传统化学显色法灵敏度高，可达pg水平，发光持续时间长，信号30min内不会发生明显降低。

组分

组分名称	数量	保存
Super ECL A	25 ml	4℃
Super ECL B	25 ml	4℃

应用

在Western Blot印迹分析，核酸杂交以及EMSA实验中，HRP标记的二抗或探针与靶分子结合再与底物反应产生可见光。

储存

2-8°C，Super ECL A要求避光保存。

实例

Fig1.检测Hela细胞裂解物的GAPDH蛋白，应用超敏ECL发光液(HaiGene,M2301)显色，一抗为兔抗GAPDH多克隆抗体，稀释比例为1:1000。

Fig2.检测A549细胞裂解物的AKT蛋白，应用超敏ECL发光液(HaiGene,M2301)和Competitor P公司的ECL发光液分别显色，
一抗为AKT rabbit mab(CST,#4691)，稀释比例为1:1000。

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超敏ECL发光液

组分

应用

储存

实例

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